Composite

Part:BBa_K3868097:Design

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)


pCBE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1572
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1572
    Illegal NheI site found at 1331
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1572
    Illegal BglII site found at 5013
    Illegal BamHI site found at 3610
    Illegal XhoI site found at 4616
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1572
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1572
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

dSpcas9 gene is from S. pyogenes for the pCas plasmid of the Crispr-Cas9 system. CDA gene is from Petromyzon marinus encoding cytosine deaminase. UGI gene is from bacteriophage PBS2 to improve the mutational efficiency.

References